User:Karmella Haynes/Notebook/PcTF Genomics/2015/01/28: Difference between revisions
From OpenWetWare
(fix raw html notebook nav) |
|||
(2 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Pc-TF Genomics</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Pc-TF Genomics</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 18: | Line 18: | ||
'''Cell plating (1/23/15)''' | '''Cell plating (1/23/15)''' | ||
* Poly lysine coating of cells (done last week) - follow protocol | * Poly-L-lysine coating of cells (done last week) - follow [http://openwetware.org/wiki/Haynes:Mamalian_IFC Mammalian IFC protocol] | ||
* Seed cells at 100k per well (aliquot in 1mL volumes into 24-well plate with poly-lysine coated cover slips on bottom) | * Seed cells at 100k per well (aliquot in 1mL volumes into 24-well plate with poly-lysine coated cover slips on bottom) | ||
* Same day - Induce cells with 1.0, 0.1, and 0.01 μg/mL dox; final volume = 2 mL | * Same day - Induce cells with 1.0, 0.1, and 0.01 μg/mL dox; final volume = 2 mL | ||
Line 37: | Line 37: | ||
* Neglected the cells, checked today out of curiosity; growth medium still healthy color | * Neglected the cells, checked today out of curiosity; growth medium still healthy color | ||
* A few cells show what look like Pc speckles in the nucleus; most others have diffuse RFP | * A few cells show what look like Pc speckles in the nucleus; most others have diffuse RFP | ||
* Fix and permeabilize these for IF staining - follow protocol | * Fix and permeabilize these for IF staining - follow [http://openwetware.org/wiki/Haynes:Mamalian_IFC Mammalian IFC protocol] | ||
Latest revision as of 00:41, 27 September 2017
Pc-TF Genomics | Main project page Previous entry Next entry |
01/28/15
Cytology of KAH126
Cell plating (1/23/15)
|