User:Karmella Haynes/Notebook/PcTF Genomics/2014/11/16: Difference between revisions
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* | * Transfection: Re-plate transfected cells | ||
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''' | '''Transfection: Re-plate transfected cells'''<br> | ||
* KAH126/MV2 cells in well 1 were ~50% - 16% RFP+ (very high!) | |||
** Could be due to low passage number (these were passage-1 cells) | |||
* Re-plated cells into two plates (U2OS complete growth medium): | |||
** 96-well: 0.2 mL per well (~5.2 E3 cells/well) | |||
** 24-well: 2.0 mL per well (~21.0 E3 cells/well) | |||
* Tomorrow (Monday), check wells for RFP signal; if confluent re-plate high RFP samples (or wait until Tuesday) | |||
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Revision as of 12:53, 17 November 2014
 Pc-TF Genomics
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11/16/14
Transfection: Re-plate transfected cells
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