User:Karmella Haynes/Notebook/PcTF Genomics/2014/10/18: Difference between revisions
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* | * Luc14 cells - Spinoculation for Jonah's samples (Biodesign) | ||
* K562 - Spinoculation (Haynes lab) | |||
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''' | '''K562 - Spinoculation (Haynes lab)'''<br> | ||
* Try spin technique on K562 cells using 15 mL conicals | |||
* Lipo LTX transfection (standard protocol) | |||
* Take half of the transfected sample (2 mL), spin at 2000 rpm for 10 min, replate | |||
* Final volumes for samples was 2mL. Did not bring up to 4 mL | |||
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Revision as of 15:10, 18 October 2014
 Pc-TF Genomics
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10/18/14
K562 - Spinoculation (Haynes lab)
--> Reaction conditions
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