User:Karmella Haynes/Notebook/PcTF Genomics/2014/09/30: Difference between revisions
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* Added 80 μL cells to each tube | * Added 80 μL cells to each tube | ||
* LaBaer lab | * LaBaer lab | ||
** | ** Biohazard set-up: Wescodyne waste and rinse; 10 mL in 2x 50 mL conicals | ||
** Added 5.0 μL 2.4 mg/mL polybrene (thawed) to each cell sample. Swirled to mix. | ** Added 5.0 μL 2.4 mg/mL polybrene (thawed) to each cell sample. Swirled to mix. | ||
** Added 400 μL virus (thawed/ spun/ pipetted to remix) to each cell sample. Swirled to mix. (rinsed pipette tip before discarding) | ** Added 400 μL virus (thawed/ spun/ pipetted to remix) to each cell sample. Swirled to mix. (rinsed pipette tip before discarding) |
Revision as of 14:50, 30 September 2014
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09/30/14
K562 transduction (trial 2)
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