User:Karmella Haynes/Notebook/PcTF Genomics/2014/09/30: Difference between revisions
From OpenWetWare
(fix raw html notebook nav) |
|||
(2 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Pc-TF Genomics</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Pc-TF Genomics</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 21: | Line 21: | ||
* Added 80 μL cells to each tube | * Added 80 μL cells to each tube | ||
* LaBaer lab | * LaBaer lab | ||
** | ** Biohazard set-up: Wescodyne waste and rinse; 10 mL in 2x 50 mL conicals | ||
** Added 5.0 μL 2.4 mg/mL polybrene (thawed) to each cell sample. Swirled to mix. | ** Added 5.0 μL 2.4 mg/mL polybrene (thawed) to each cell sample. Swirled to mix. | ||
** Added 400 μL virus (thawed/ spun/ pipetted to remix) to each cell sample. Swirled to mix. (rinsed pipette tip before discarding) | ** Added 400 μL virus (thawed/ spun/ pipetted to remix) to each cell sample. Swirled to mix. (rinsed pipette tip before discarding) | ||
***Note: had some left-over virus; marked these with black dot on caps, placed back into the -80°C box | ***Note: had some left-over virus; marked these with black dot on caps, placed back into the -80°C box | ||
** Spun at 2250 | ** Spun at 2250 rpm (1178 rcf) for 25 min. | ||
** Pipetted u/d to resuspend, transferred cells to 6 mL plate | ** Pipetted u/d to resuspend, transferred cells to 6 mL plate | ||
** Grew at 37°C. Will check GFP expression tomorrow. | ** Grew at 37°C. Will check GFP expression tomorrow. |
Latest revision as of 00:21, 27 September 2017
Pc-TF Genomics | Main project page Previous entry Next entry |
09/30/14
K562 transduction (trial 2)
|