User:Karmella Haynes/Notebook/PcTF Genomics/2014/09/30: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Pc-TF Genomics</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Pc-TF Genomics</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==09/30/14== | ||
<!-- Precede finished items with a checkmark ✓ --> | <!-- Precede finished items with a checkmark ✓ --> | ||
* | * K562 transduction (trial 2) | ||
* Cell culture: passaged U2OS 1:10, K562 1:10 | |||
* Cell culture: started Luc14 & Gal4EED/luc cultures (4 mL 20% FBS/ 1% P/S) | |||
---- | ---- | ||
''' | '''K562 transduction (trial 2)'''<br> | ||
* See ##/##/## for transgene/virus list | |||
* Cell stock passaged 1:10 on 9/22/14. Assume 1.0E6 cells/mL | |||
* Added 1 mL complete culture medium to 6x 15 mL conicals | |||
* Added 80 μL cells to each tube | |||
* LaBaer lab | |||
** Biohazard set-up: Wescodyne waste and rinse; 10 mL in 2x 50 mL conicals | |||
** Added 5.0 μL 2.4 mg/mL polybrene (thawed) to each cell sample. Swirled to mix. | |||
** Added 400 μL virus (thawed/ spun/ pipetted to remix) to each cell sample. Swirled to mix. (rinsed pipette tip before discarding) | |||
***Note: had some left-over virus; marked these with black dot on caps, placed back into the -80°C box | |||
** Spun at 2250 rpm (1178 rcf) for 25 min. | |||
** Pipetted u/d to resuspend, transferred cells to 6 mL plate | |||
** Grew at 37°C. Will check GFP expression tomorrow. | |||
Latest revision as of 00:21, 27 September 2017
Pc-TF Genomics | Main project page Previous entry Next entry |
09/30/14
K562 transduction (trial 2)
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