User:Karmella Haynes/Notebook/PcTF Genomics/2014/09/25: Difference between revisions
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* | * Transduction Day 3: expand cells | ||
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''' | '''Transduction Day 3'''<br> | ||
* Brought to LaBaer lab: trypsin EDTA, 1x PBS, bottles of media for U2OS and SK-N-SH, 10 cm plates | |||
* Checked growth of cells: | |||
** U2OS '''ready to expand''' (~80% confluent) | |||
** SK-N-SH not ready to expand (left in incubator) | |||
** K562 - still no visible expression in control well | |||
* Harvested and expanded U2OS cells in 10 mL medium in 10 cm plates (6 plates total) | |||
* Grow at 37°C (overnight) | |||
Revision as of 07:06, 28 September 2014
 Pc-TF Genomics
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09/25/14
Transduction Day 3
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