User:Karmella Haynes/Notebook/PcTF Genomics/2014/09/25: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Pc-TF Genomics</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Pc-TF Genomics</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==mm/dd/yy==
==09/25/14==
<!-- Precede finished items with a checkmark &#x2713; -->
<!-- Precede finished items with a checkmark &#x2713; -->
* Line item 1
* Transduction Day 3: expand cells
 


----
----
'''Line item 1'''<br>
'''Transduction Day 3'''<br>
> Samples
* Brought to LaBaer lab: trypsin EDTA, 1x PBS, bottles of media for U2OS and SK-N-SH, 10 cm plates
 
* Checked growth of cells:
{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table -->
** U2OS '''ready to expand''' (~80% confluent)
|-valign="top"
** SK-N-SH not ready to expand (left in incubator)
| <u>Reagent</u> || <u>Volume</u>
** K562 - still no visible expression in control well
|-
* Harvested and expanded U2OS cells in 10 mL medium in 10 cm plates (6 plates total)
| reagent 1 || # μL
* Grow at 37°C (overnight)
|-
| reagent 2 || #
|-
| reagent 3 || #
|-
| reagent 4 || #
|-
| dH<sub>2</sub>O || #
|-
| &nbsp; || # μL
|}
 
--> Reaction conditions




Latest revision as of 00:21, 27 September 2017

Pc-TF Genomics Main project page
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09/25/14

  • Transduction Day 3: expand cells


Transduction Day 3

  • Brought to LaBaer lab: trypsin EDTA, 1x PBS, bottles of media for U2OS and SK-N-SH, 10 cm plates
  • Checked growth of cells:
    • U2OS ready to expand (~80% confluent)
    • SK-N-SH not ready to expand (left in incubator)
    • K562 - still no visible expression in control well
  • Harvested and expanded U2OS cells in 10 mL medium in 10 cm plates (6 plates total)
  • Grow at 37°C (overnight)



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