User:Karmella Haynes/Notebook/PcTF Genomics/2014/09/24: Difference between revisions

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(Autocreate 2014/09/24 Entry for User:Karmella_Haynes/Notebook/PcTF_Genomics)
 
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==mm/dd/yy==
==09/24/14==
<!-- Precede finished items with a checkmark &#x2713; -->
<!-- Precede finished items with a checkmark &#x2713; -->
* Line item 1
* Transduction Day 2: change media


----
----
'''Line item 1'''<br>
'''Transduction Day 2'''<br>
> Samples
* Check GFP controls
 
** Plate 1: U2OS, ~90% GFP expression, at least ~two doubling
{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table -->
** Plate 2: SK-N-SH, ~90% expression, slow cell growth
|-valign="top"
** Plate 4: K562, <font color="red">no GFP expression</font>
| <u>Reagent</u> || <u>Volume</u>  
* Remove media
|-
* Add 2 ml/well fresh media with 1% Pen/strep
| reagent 1 || # μL
* Grow cells at 37°C (overnight)
|-
| reagent 2 || #
|-
| reagent 3 || #
|-
| reagent 4 || #
|-
| dH<sub>2</sub>O || #
|-
| &nbsp; || # μL
|}
 
--> Reaction conditions
 




Revision as of 06:56, 28 September 2014

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09/24/14

  • Transduction Day 2: change media

Transduction Day 2

  • Check GFP controls
    • Plate 1: U2OS, ~90% GFP expression, at least ~two doubling
    • Plate 2: SK-N-SH, ~90% expression, slow cell growth
    • Plate 4: K562, no GFP expression
  • Remove media
  • Add 2 ml/well fresh media with 1% Pen/strep
  • Grow cells at 37°C (overnight)



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