User:Karmella Haynes/Notebook/PcTF Genomics/2014/09/23: Difference between revisions
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'''Transductions'''<br> | '''Transductions'''<br> | ||
Note: cells are in standard complete medium | |||
# Plate 1: U2OS | # Plate 1: U2OS | ||
# Plate 2: SK-N-SH | # Plate 2: SK-N-SH | ||
# Plate 4: K562 b (1 mL) - no one was around to consult on whether I should pellet the 2mL and replate | # Plate 4: K562 b (1 mL) - no one was around to consult on whether I should pellet the 2mL and replate | ||
Wells (for all plates) - added 400 μL virus | |||
# well 1 - GFP | |||
# well 2 - KAH160/015 (2010) | |||
# well 3 - KAH160/015 (2014) | |||
# well 4 - KAH165/015 (2010) | |||
# well 6 - KAH165/015 (2014) | |||
# well 7 - KAH170/015 (2014) | |||
Abbreviated protocol (from Seron Eaton) | Abbreviated protocol (from Seron Eaton) | ||
* | * Retrieve frozen aliquots of harvested virus from -80C freezer (just outside TC room) | ||
* Thaw and quick-spin to get virus off of the inside of lid | |||
* Biohazard set-up: 10 mL Wescodyne waste conical (50 mL), 10 mL Wescodyne rinse | |||
Revision as of 06:40, 28 September 2014
 Pc-TF Genomics
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09/23/14
Transductions
Wells (for all plates) - added 400 μL virus
Abbreviated protocol (from Seron Eaton)
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