User:Karmella Haynes/Notebook/PcTF Genomics/2014/09/22: Difference between revisions
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* | * 6-well plates for transductions | ||
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''' | '''Cultures for Transduction'''<br> | ||
# Plate 1: U2OS | |||
# Plate 2: SK-N-SH | |||
# Plate 3: K562 a (suspension) | |||
# Plate 4: K562 b (suspension) | |||
* Plated at ~40,000 cells per well | |||
** Diluted 100% confluent 10 mL harvested cells as follows: 0.3 mL in 15 mL medium | |||
* 2 mL media per well | |||
* For K562... | |||
** a: made a 40k cell/ 2 mL / well plate | |||
** b: made a 40k cell/ 1mL / well plate - transduction protocol calls for removal of 1mL medium prior to transduction. Set up this plate to avoid having to remove the 1 mL from suspended cells | |||
Revision as of 06:22, 28 September 2014
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09/22/14
Cultures for Transduction
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