User:Karmella Haynes/Notebook/PcTF Genomics/2014/09/10: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Pc-TF Genomics</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Pc-TF Genomics</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==09/10/14== | ||
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* | * Lenti Transfection: Day 2 - change out medium (LaBaer lab) | ||
---- | ---- | ||
''' | '''Lenti Transfection: Day 2 - change out medium'''<br> | ||
> | Note: At this point, cells are producing virus. Follow extra biosafety precautions.<br> | ||
PPE note: Wear 2x pairs of gloves. Dispose of and replace outer gloves as needed. | |||
* Medium change 1 - 10:00 am | |||
# In the BS cabinet, set up 2x 50 mL conicals of 10 mL of 10x Wescodyne (brown) germicidal detergent. Use one for discarding viral medium, one for rinsing pipettes. | |||
# Checked GFP control (sample 7) under scope. Nearly 100% expression. (Note: scope had no RFP filter set) | |||
# For each well (one at a time)... | |||
## '''Removed 1.5 mL growth medium''', dispensed into Wescodyne, rinsed pipette in Wesc., discarded pipette | |||
## Slowly and carefully '''added 2 mL fresh growth medium''', rinsed pipette in Wesc., discarded pipette | |||
## Final volume ~3 mL | |||
# Placed plates back into 37°C incubator. | |||
# Let discarded medium/Wesc. sit for 15 min. | |||
# Wiped down BS cabinet surface with Microbiocide. | |||
# Aspirated off discarded waste, then rinse solution. | |||
-- | * Medium change 2 - 4:00 pm | ||
# Same biosafety/ discard set-up as before | |||
# For each well (one at a time)... | |||
## '''Removed 2.5 mL growth medium''', dispensed into Wescodyne, rinsed pipette in Wesc., discarded pipette | |||
## Slowly and carefully '''added 5.5 mL fresh growth medium''', rinsed pipette in Wesc., discarded pipette | |||
# Placed plates back into 37°C incubator. | |||
# Same biosafety/ discard procedure as before | |||
Latest revision as of 00:16, 27 September 2017
Pc-TF Genomics | Main project page Previous entry Next entry |
09/10/14
Lenti Transfection: Day 2 - change out medium
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