User:Karmella Haynes/Notebook/PcTF Genomics/2014/06/04: Difference between revisions
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* Do a new RNA extraction from TRIzol samples | * Do a new RNA extraction from TRIzol samples | ||
'''RNA extraction''' | |||
* Follow the TRIzol/ RNeasy protocol | |||
* Use Carly's TRIzol samples K562 1, 2, 3, and 4 | |||
'''Measure RNA concentration'''<br> | |||
{| class="wikitable" border="0" cellspacing="5" | |||
|- | |||
| <u>Sample</u> || <u>OD 260</u> || <u>260/280</u> || <u>ng/μL</u> || <u>Vol. to use for cDNA synth.</u> | |||
|- | |||
| 1. K562 1 || ### || ### || ### || ### μL (### μg) | |||
|- | |||
| 2. K562 2 || ### || ### || ### || ### μL (### μg) | |||
|- | |||
| 2. K562 3 || ### || ### || ### || ### μL (### μg) | |||
|- | |||
| 2. K562 4 || ### || ### || ### || ### μL (### μg) | |||
|} | |||
Revision as of 17:12, 4 June 2014
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06/04/14
cDNA synthesis: K562
RNA extraction
Measure RNA concentration
oligo(dT) Primer annealing
--> Incubate at 65°C/ 5 min. Immediately place on ice for 1 min.
--> Aliquot 10 μL of mix into 8-tube strip
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