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Pc-TF Genomics
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01/27/14
- qRT-PCR experiment - PlateK562_1/ U2OS
qRT-PCR - Plate K562_1/ U2OS
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Template
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Gene Target
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Rxn 1: |
treated cells |
KIT, primers 13/14
|
Rxn 2: |
treated cells |
TNFRSF11A, primers 15/16
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Rxn 3: |
treated cells |
EGFR, primers 17/18
|
Rxn 4: |
treated cells |
WT1, primers 19/20
|
Rxn 5: |
treated cells |
HLF, primer 21/22
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Rxn 6: |
treated cells |
BCL6, primer 23/24
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Rxn 7: |
treated cells |
mCh
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Rxn 8: |
treated cells |
ref. gene, GAPD
|
Rxn 9: |
untreated cells |
KIT, primers 13/14
|
Rxn 10: |
untreated cells |
TNFRSF11A, primers 15/16
|
Rxn 11: |
untreated cells |
EGFR, primers 17/18
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Rxn 12: |
untreated cells |
WT1, primers 19/20
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Rxn 13: |
untreated cells |
HLF, primer 21/22
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Rxn 14: |
untreated cells |
BCL6, primer 23/24
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Rxn 15: |
untreated cells |
mCh
|
Rxn 16: |
untreated cells |
ref. gene, GAPD
|
Rxn 17: |
no template |
KIT, primers 13/14
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Rxn 18: |
no template |
TNFRSF11A, primers 15/16
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Rxn 19: |
no template |
EGFR, primers 17/18
|
Rxn 20: |
no template |
WT1, primers 19/20
|
Rxn 21: |
no template |
HLF, primer 21/22
|
Rxn 22: |
no template |
BCL6, primer 23/24
|
Rxn 23: |
no template |
mCh
|
Rxn 24: |
no template |
ref. gene, GAPD
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Primer/Probe Master Mixes (8 total)
Reagent
|
(Single well)
|
Gene Target 1 - 7 (x10)
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Gene Target GAPD (x10)
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2x LC480 Probes Master |
(7.5 μL) |
75 |
75
|
20 μM Forward primer |
(0.3 μL) |
3 |
3.0 GAPD primers*
|
20 μM Reverse primer |
(0.3 μL) |
3 |
---
|
10 μM UPL probe |
(0.3 μL) |
3 |
3.0 GAPD UPL probe*
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PCR H2O |
(0.1 μL) |
1 |
4
|
Total vol. |
(8.5 μL) |
85 |
85
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