User:Karmella Haynes/Notebook/PcTF Genomics/2014/01/27: Difference between revisions
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| Total vol.||(8.5 μL)||85||85 | | Total vol.||(8.5 μL)||85||85 | ||
|} | |} | ||
'''Template Master Mixes''' | |||
* Use 1:10 dilution of cDNA for targets | |||
* Use 1:100 dilution of cDNA for GAPD | |||
{| | |||
| align="center" style="background:#f0f0f0;"|'''Reagent''' | |||
| align="center" style="background:#f0f0f0;"|'''(Single well)''' | |||
| align="center" style="background:#f0f0f0;"|'''treated cDNA Template (x25)''' | |||
| align="center" style="background:#f0f0f0;"|'''untreated cDNA Template (x25)''' | |||
| align="center" style="background:#f0f0f0;"|'''no Template (x25)''' | |||
|- | |||
| diluted cDNA||(2.0 μL)||50||50||--- | |||
|- | |||
| PCR H2O||(4.5 μL)||112.5||112.5||162.5 | |||
|- | |||
| Total vol.||(6.5 μL)||162.5||162.5||162.5 | |||
|} | |||
* 96-well plate | |||
** First of each triplicate well, add 25.5 Primer/Probe MM + 19.5 Template MM | |||
Revision as of 16:58, 24 January 2014
Pc-TF Genomics | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
01/27/14
qRT-PCR - Plate K562_1/ U2OS
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