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 Pc-TF Genomics
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07/05/13
- HEK293 Gal4-EED transfection - for PcTF expression and flow cytometry
Lipo transfection
> Samples are for flow cytometry
> Seed new 6-well plates w/ ~5x105 cells/well in 3.4 mL plain medium (don't have any p.s.-free)
> Transfect human PcTF under control of constitutive CMV-TetO promoter (KAH160/MV1); 347 ng/μL; Note: this cell line has TetR, so constitutive CMV is needed.
| Wells |
Plasmid |
DNA |
Volume + dH2O |
Opti-MEM |
PLUS |
Lipo |
|
| 1 |
KAH160/MV1 |
2 μg |
8.8 μL +11.2 |
570 μL |
2.5 μL |
7.5 μL
|
| 2 |
KAH160/MV1 |
2 μg |
8.8 μL +11.2 |
" |
" |
"
|
| 3 |
mock |
none |
0 μL + 20.0 |
" |
" |
"
|
| 4 |
none |
none |
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Pc-TF Genomics
