mm/dd/yy
- Gl4-EED/luc time/dose - luc assay & cell counts
Luciferase activity assay - time point: 4 days
Cell prep
- Harvested cells (induced on 6/30/13)
- Seeded new 2 new plates with non-induced cells from 6/30/13
- Pelleted all of the dox+ cells, resuspended in 2 mL FACS buffer
Assay reagents
- Used the Biotium Steady-Luc Firefly HTS Assay Kit (same as 6/18/13)
Luc assay
- Filtered 700 μL cells through strainer caps
- Used opaque white Costar plate (from Rege lab)
- Samples loaded in triplicate (by columns)
- Included luc buffer + FACS-buffer "blank" sample (well D1)
- Other steps same as 6/18/13
Cell counts
- Wang lab's Accuri flow cytometer.
- Set machine to read 20 uL of cells
- Be sure to "clean" with water-run in between samples
Sample ID |
Gated count/ 20 μL |
|
Cells/ 100 μL
|
sample 1 |
### |
x5 = |
54,400
|
sample 2 |
10,430 |
x5 = |
52,150
|
sample 3 |
11,897 |
x5 = |
59,485
|
sample 4 |
10,316 |
x5 = |
51,580
|
sample 5 |
9,784 |
x5 = |
48,920
|
sample 6 |
11,549 |
x5 = |
57,745
|
|