User:Karmella Haynes/Notebook/PcTF Genomics/2013/07/04: Difference between revisions
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''' | '''Luciferase activity assay - time point: 4 days'''<br> | ||
> | |||
<u>Cell prep</u> | |||
* Harvested cells (induced on 6/30/13) | |||
** Seeded new 2 new plates with non-induced cells from 6/30/13 | |||
** Pelleted all of the dox+ cells, resuspended in 2 mL FACS buffer | |||
<u>Assay reagents</u> | |||
* Used the Biotium Steady-Luc Firefly HTS Assay Kit (same as 6/18/13) | |||
<u>Luc assay</u> | |||
* Filtered '''700 μL cells''' through strainer caps | |||
* Other steps same as 6/18/13 | |||
<u>Cell counts</u> | |||
* Wang lab's Accuri flow cytometer. | |||
* Set machine to read 20 uL of cells | |||
* Be sure to "clean" with water-run in between samples | |||
{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table --> | {| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table --> | ||
|-valign="top" | |-valign="top" | ||
| <u> | | <u>Sample ID</u> || <u>Gated count/ 20 μL</u> || || <u>Cells/ 100 μL</u> | ||
|- | |- | ||
| | | sample 1 || 10,880 || x5 = || 54,400 | ||
|- | |- | ||
| | | sample 2 || 10,430 || x5 = || 52,150 | ||
|- | |- | ||
| | | sample 3 || 11,897 || x5 = || 59,485 | ||
|- | |- | ||
| | | sample 4 || 10,316 || x5 = || 51,580 | ||
|- | |- | ||
| | | sample 5 || 9,784 || x5 = || 48,920 | ||
|- | |- | ||
| | | sample 6 || 11,549 || x5 = || 57,745 | ||
|} | |} | ||
Revision as of 19:41, 4 July 2013
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mm/dd/yy
Luciferase activity assay - time point: 4 days
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