User:Karmella Haynes/Notebook/PcTF Genomics/2013/07/04: Difference between revisions
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==mm/dd/yy== | ==mm/dd/yy== | ||
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* | * Gl4-EED/luc time/dose - luc assay & cell counts | ||
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<u>Luc assay</u> | <u>Luc assay</u> | ||
* Filtered '''700 μL cells''' through strainer caps | * Filtered '''700 μL cells''' through strainer caps | ||
* Used opaque white Costar plate (from Rege lab) | |||
* Samples loaded in triplicate (by columns) | |||
* Included luc buffer + FACS-buffer "blank" sample (well D1) | |||
* Other steps same as [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2013/07/04 6/18/13] | * Other steps same as [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2013/07/04 6/18/13] | ||
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| <u>Sample ID</u> || <u>Gated count/ 20 μL</u> || || <u>Cells/ 100 μL</u> | | <u>Sample ID</u> || <u>Gated count/ 20 μL</u> || || <u>Cells/ 100 μL</u> | ||
|- | |- | ||
| sample 1 || | | sample 1 || ### || x5 = || 54,400 | ||
|- | |- | ||
| sample 2 || 10,430 || x5 = || 52,150 | | sample 2 || 10,430 || x5 = || 52,150 |
Revision as of 20:14, 4 July 2013
Pc-TF Genomics | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||
mm/dd/yy
Luciferase activity assay - time point: 4 days
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