User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/30: Difference between revisions
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> Cells were 100% confluent<br> | > Cells were 100% confluent<br> | ||
> Re-plate 1:2 dilution prior to transfection in 3.4 mL p/s-free medium<br> | > Re-plate 1:2 dilution prior to transfection in 3.4 mL p/s-free medium<br> | ||
> Transfect human PcTF under control of constitutive CMV-TetO promoter (KAH126/MV2); 226 ng/μL | > Transfect human PcTF under control of constitutive CMV-TetO promoter (KAH126/MV2); 226 ng/μL; Note: no TetR in SK-N-SH, so expression will be constitutive | ||
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| 2 || KAH126/MV2 || 2 μg || 8.8 μL +11.2 || " || " || " | | 2 || KAH126/MV2 || 2 μg || 8.8 μL +11.2 || " || " || " | ||
|- | |- | ||
| 3 || KAH126/MV2 || 2 μg || 8.8 μL +11.2 || " || " || " | | 3 || <s>KAH126/MV2</s>* || 2 μg || 8.8 μL +11.2 || " || " || " | ||
|- | |- | ||
| 4 || mock || none || 0 μL + 20.0 || " || " || " | | 4 || mock || none || 0 μL + 20.0 || " || " || " | ||
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| 6 || mock || none || 0 μL + 20.0 || " || " || " | | 6 || mock || none || 0 μL + 20.0 || " || " || " | ||
|} | |} | ||
* Ran out of plasmid DNA | |||
> Add 570 μL Opti-MEM to each 20 μL DNA sample <br> | > Add 570 μL Opti-MEM to each 20 μL DNA sample <br> |
Revision as of 15:18, 30 June 2013
Pc-TF Genomics | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6/30/13/dd/yy
Gal4-EED/luc cells IFC
Addition of serial dilutions of dox to cells
Lipo transfection
> Add 570 μL Opti-MEM to each 20 μL DNA sample
|