User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/30: Difference between revisions
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# 10<sup>-4</sup> | # 10<sup>-4</sup> | ||
# 0 | # 0 | ||
Addition of serial dilutions of dox to cells | |||
* Start with 3.5 mL growth medium in cell culture wells. Remove some growth medium from cells where necessary. | |||
* Set up the following serial dilutions (using appropriate growth medium) in sterile 1 mL tubes... | |||
{| {{table}} | |||
|- | |||
| || tube 1 || 2 || 3 || 4 || 5 || 6 | |||
|- | |||
| 1 mg/mL dox (μL) || 8.0 || --- || --- || --- || --- || --- | |||
| - | |||
| mixture from prior tube (μL) || --- || 100 || 100 || 100 || 100 || --- | |||
|- | |||
| +growth med. (μL) || 1000 || 900 || 900 || 900 || 900 || --- | |||
|- | |||
| Final [dox] (μg/mL) in 4 mL med. || 1.0 || 10<sup>-1</sup> || 10<sup>-2</sup> || 10<sup>-3</sup> || 10<sup>-4</sup> || 0 | |||
|} | |||
Revision as of 15:16, 30 June 2013
Pc-TF Genomics | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6/30/13/dd/yy
Gal4-EED/luc cells IFC
Addition of serial dilutions of dox to cells
Lipo transfection
> Add 570 μL Opti-MEM to each 20 μL DNA sample
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