User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/26: Difference between revisions
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* Step 5 - Create binding proteins: created "H3K27me3" for 6_aln_sorted. | * Step 5 - Create binding proteins: created "H3K27me3" for 6_aln_sorted. | ||
* Step 6 - Assigned H3K27me3 as protein label for 6_aln_sorted; Set control inputU2OS as "yes" for "Is control?"; Set inputU2OS as control for 6_aln_sorted. | * Step 6 - Assigned H3K27me3 as protein label for 6_aln_sorted; Set control inputU2OS as "yes" for "Is control?"; Set inputU2OS as control for 6_aln_sorted. | ||
* Step 11 - Chose BED files only (wigs take up lots of disk space) | |||
* Step 12 - Deselected "Directionality" | * Step 12 - Deselected "Directionality" | ||
Revision as of 21:14, 27 June 2013
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06/26/13
ChIP seq analysis with Input control Array Star analysis - general instructions
H3K27me3 enrichment in U2OS
U2OS TRIzol prep
(Should provide more than enough RNA. Discarded other cultures)
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