User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/18: Difference between revisions
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* | * Luciferase activity assay | ||
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''' | '''Luciferase activity assay - time point 1'''<br> | ||
> | * Harvested cells | ||
** Seeded new dox+ plate with 1/2 of cells | |||
** Pelleted other half, resuspended in 2 mL FACS buffer | |||
* Used the Biotium Steady-Luc Firefly HTS Assay Kit | |||
* 6 experimental samples x 3 repliates = '''18 assays (one time point)''' | |||
* Need 100 μL complete steady-luc buffer per assay = '''1800 μL complete steady-luc buffer''' | |||
* Dissolve dry D-luciferin to make 50x stock by adding 80 μL dH<sub>2</sub>O to the vial | |||
* Make 2000 μL complete steady-luc buffer: Add 40 μL 50x D-luciferin to 2 mL buffer | |||
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Revision as of 17:51, 18 June 2013
 Pc-TF Genomics
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06/18/13
Luciferase activity assay - time point 1
--> Reaction conditions
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