User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/18

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(Autocreate 2013/06/18 Entry for User:Karmella_Haynes/Notebook/PcTF_Genomics)
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==06/18/13==
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* Line item 1
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* Luciferase activity assay
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'''Line item 1'''<br>
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'''Luciferase activity assay - time point 1'''<br>
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> Samples
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* Harvested cells
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** Seeded new dox+ plate with 1/2 of cells
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** Pelleted other half, resuspended in 2 mL FACS buffer
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* Used the Biotium Steady-Luc Firefly HTS Assay Kit
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* 6 experimental samples x 3 repliates = '''18 assays (one time point)'''
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* Need 100 μL complete steady-luc buffer per assay = '''1800 μL complete steady-luc buffer'''
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* Dissolve dry D-luciferin to make 50x stock by adding 80 μL dH<sub>2</sub>O to the vial
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* Make 2000 μL complete steady-luc buffer: Add 40 μL 50x D-luciferin to 2 mL buffer
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Revision as of 20:51, 18 June 2013

Pc-TF Genomics Main project page
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06/18/13

  • Luciferase activity assay



Luciferase activity assay - time point 1

  • Harvested cells
    • Seeded new dox+ plate with 1/2 of cells
    • Pelleted other half, resuspended in 2 mL FACS buffer
  • Used the Biotium Steady-Luc Firefly HTS Assay Kit
  • 6 experimental samples x 3 repliates = 18 assays (one time point)
  • Need 100 μL complete steady-luc buffer per assay = 1800 μL complete steady-luc buffer
  • Dissolve dry D-luciferin to make 50x stock by adding 80 μL dH2O to the vial
  • Make 2000 μL complete steady-luc buffer: Add 40 μL 50x D-luciferin to 2 mL buffer


Reagent Volume
reagent 1 # μL
reagent 2 #
reagent 3 #
reagent 4 #
dH2O #
  # μL

--> Reaction conditions



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