User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/15: Difference between revisions
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* Gal4-EED/luc Luciferase ICC - fixation, permeabilization, primary ab staining | * Gal4-EED/luc Luciferase ICC - fixation, permeabilization, primary ab staining | ||
* Gal4-EED/ luc dox induction - shipment 2 cells | |||
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* At the primary antibody staining step, keep samples and reagents on ice overnight in the cold room | * At the primary antibody staining step, keep samples and reagents on ice overnight in the cold room | ||
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'''Gal4-EED/luc dox induction'''<br> | |||
Samples (Shipment 2 cells); new 6-well plate... | |||
# 0 μg/mL dox | |||
# 0.1 | |||
# 0.2 | |||
# 0.5 | |||
# 1.0 | |||
# 2.0 (induced on 6/02/13, 13 days) | |||
* For this set, test earlier time points and try luc activity assay | |||
Revision as of 18:02, 17 June 2013
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06/15/13
Gal4-EED/luc Luciferase ICC Samples (Shipment 1 cells)
Gal4-EED/luc dox induction Samples (Shipment 2 cells); new 6-well plate...
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