User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/15

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* Gal4-EED/luc Luciferase ICC - fixation, permeabilization, primary ab staining
* Gal4-EED/luc Luciferase ICC - fixation, permeabilization, primary ab staining
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# 2.0
# 2.0
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* Harvested cells from one 6-well plate (split plate #2 for further analyses)
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* At the primary antibody staining step, keep samples and reagents on ice overnight in the cold room

Revision as of 19:57, 17 June 2013

Pc-TF Genomics Main project page
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06/15/13

  • Gal4-EED/luc Luciferase ICC - fixation, permeabilization, primary ab staining



Gal4-EED/luc Luciferase ICC

Samples

  1. 0 μg/mL dox
  2. 0.1
  3. 0.2
  4. 0.5
  5. 1.0
  6. 2.0
  • Harvested cells from one 6-well plate (split plate #2 for further analyses)
  • At the primary antibody staining step, keep samples and reagents on ice overnight in the cold room



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