User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/15: Difference between revisions

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'''Gal4-EED/luc Luciferase ICC'''<br>
'''Gal4-EED/luc Luciferase ICC'''<br>


Samples
Samples (Shipment 1 cells)
# 0 μg/mL dox
# 0 μg/mL dox
# 0.1  
# 0.1  

Revision as of 17:58, 17 June 2013

Pc-TF Genomics <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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06/15/13

  • Gal4-EED/luc Luciferase ICC - fixation, permeabilization, primary ab staining


Gal4-EED/luc Luciferase ICC

Samples (Shipment 1 cells)

  1. 0 μg/mL dox
  2. 0.1
  3. 0.2
  4. 0.5
  5. 1.0
  6. 2.0
  • Harvested cells from one 6-well plate (split plate #2 for further analyses)
  • At the primary antibody staining step, keep samples and reagents on ice overnight in the cold room



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