User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/13: Difference between revisions
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* | * Gal4-EED/luc cells IFC - secondary stain & imaging | ||
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''' | '''Gal4-EED/luc cells IFC'''<br> | ||
* Samples: first row of wells in the 24-well plate. | |||
* Keep all others under PBS | |||
# 0 μg/mL dox | |||
# 0.1 | |||
# 0.2 | |||
# 0.5 | |||
# 1.0 | |||
# 2.0 | |||
* Follow standard protocol; wash 3x with 1xPBS | |||
* Use 1:1000 dilution of anti-rabbit Alexa488 and 1:1000 Hoescht (in 15 μL Gentex horse serum blocking solution per well) | |||
* Incubate under parafilm "covers" for one hour at room temperature in light-tight box with wet paper towels | |||
* Use GFP setting on Nikon scope to record images | |||
Results: | |||
* Saw some cells with very bright signal, some dim in 0 μg/mL dox well | |||
* Signal appeared to be dimmer overall for all others. | |||
Revision as of 17:46, 17 June 2013
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06/13/13
Gal4-EED/luc cells IFC
Results:
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