User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/12: Difference between revisions
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* Use 1:200 dilution of rabbit (poly) anti-luc | * Use 1:200 dilution of rabbit (poly) anti-luc (in 15 μL Gentex horse serum blocking solution per well) | ||
* Incubate under parafilm "covers" overnight at 4°C | * Incubate under parafilm "covers" overnight at 4°C in box with wet paper towels | ||
Revision as of 21:29, 12 June 2013
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06/12/13
Gal4-EED/luc cells IFC
Lipo transfection
> Add 570 μL Opti-MEM to each 20 μL DNA sample
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