User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/11

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(Autocreate 2013/06/11 Entry for User:Karmella_Haynes/Notebook/PcTF_Genomics)
Current revision (22:18, 12 June 2013) (view source)
(06/11/13)
 
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==mm/dd/yy==
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==06/11/13==
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* Line item 1
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* Gal4-EED/luc HEK +dox cytology: fix and permeabilize cells
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'''Line item 1'''<br>
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'''Gal4-EED/luc HEK +dox cytology'''<br>
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> Samples
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> Use 24-well plate from 6/10/13
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{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table -->
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|-valign="top"
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| <u>Reagent</u> || <u>Volume</u>
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|-
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| reagent 1 || # μL
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|-
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| reagent 2 || #
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|-
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| reagent 3 || #
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|-
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| reagent 4 || #
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|-
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| dH<sub>2</sub>O || #
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|-
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| &nbsp; || # μL
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|}
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--> Reaction conditions
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* Use David Dreher's protocol, except dilute dry paraformaldehyde to make fresh fixing buffer.
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* Process all 24 wells
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06/11/13

  • Gal4-EED/luc HEK +dox cytology: fix and permeabilize cells



Gal4-EED/luc HEK +dox cytology
> Use 24-well plate from 6/10/13

  • Use David Dreher's protocol, except dilute dry paraformaldehyde to make fresh fixing buffer.
  • Process all 24 wells


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