User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/11

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Current revision (22:18, 12 June 2013) (view source)
(06/11/13)
 
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==06/11/13==
==06/11/13==
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* Gal4-EED/luc HEK +dox cytology: fix and stain cells with primary ab
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* Gal4-EED/luc HEK +dox cytology: fix and permeabilize cells
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* Use David Dreher's protocol, except dilute dry paraformaldehyde to make fresh fixing buffer.
* Use David Dreher's protocol, except dilute dry paraformaldehyde to make fresh fixing buffer.
 +
* Process all 24 wells
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06/11/13

  • Gal4-EED/luc HEK +dox cytology: fix and permeabilize cells



Gal4-EED/luc HEK +dox cytology
> Use 24-well plate from 6/10/13

  • Use David Dreher's protocol, except dilute dry paraformaldehyde to make fresh fixing buffer.
  • Process all 24 wells


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