User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/10: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Pc-TF Genomics</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Pc-TF Genomics</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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* Split cells: Gal4-EED/luc shipment 1 and shipment 2 T-75 flasks (1:5) | * Split cells: Gal4-EED/luc shipment 1 and shipment 2 T-75 flasks (1:5) | ||
* Split dox induced cells | * Split dox induced cells | ||
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24-well plate for microscopy: | 24-well plate for microscopy: | ||
* Use leftover suspension from 6-well plating | * Use leftover suspension from 6-well plating | ||
* For each sample (1-6) add 0.5 mL suspension to 0.5 mL fresh medium +proper amount of | * For each sample (1-6) add 0.5 mL suspension to 0.5 mL fresh medium +proper amount of dox in 4 wells | ||
* Cells will be fixed and stained directly in the plate, following David Dreher's protocol. | * Cells will be fixed and stained directly in the plate, following David Dreher's protocol. | ||
Latest revision as of 22:45, 26 September 2017
Pc-TF Genomics | Main project page Previous entry Next entry | |||||||||||||||||||||
06/10/13
Gal4EED/luc test
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