User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/03: Difference between revisions
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* Ran samples 6 (blank), 1, 2, and 3 and recorded SS, FS, and mCherry | * Ran samples 6 (blank), 1, 2, and 3 and recorded SS, FS, and mCherry | ||
* Very modest mCh over background (blank). Tong noted that the mCherry laser was not optimal for this fluorophore. | * Very modest mCh over background (blank). Tong noted that the mCherry laser was not optimal for this fluorophore. | ||
** ~1% RFP+ over background (with gating) | |||
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* Drawback is that voltage/ sensitivity cannot be adjusted, so blank signal is in the 10<sup>3</sup> range. | * Drawback is that voltage/ sensitivity cannot be adjusted, so blank signal is in the 10<sup>3</sup> range. | ||
* Do further tests to see if Accuri GFP settings can detect CFP (AmCyan). | * Do further tests to see if Accuri GFP settings can detect CFP (AmCyan). | ||
Revision as of 15:48, 11 June 2013
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06/03/13
Flow cytometry at Biodesign Samples:
Harvesting:
Flow cytometry:
Flow cytometry - Wang Lab's Accuri C6
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