User:Karmella Haynes/Notebook/PcTF Genomics/2013/01/25: Difference between revisions
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--> 15 min./ r.t.<br> | --> 15 min./ r.t.<br> | ||
--> Zymo clean and concentrator, elute with 10 μL st.dH<sub>2</sub>O<br> | --> Zymo clean and concentrator, elute with 10 μL st.dH<sub>2</sub>O<br> | ||
--> Store at -20°C | |||
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Day 2 (1/26/13)<br> | |||
<u>Size Select the Library</u><br> | <u>Size Select the Library</u><br> | ||
> Run all samples (+ loading buffer) in a 2% TAE gel<br> | > Run all samples (+ loading buffer) in a 2% TAE gel<br> |
Revision as of 18:13, 26 January 2013
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01/25/13
Illumina ChIP-seq prep
Perform End Repair
--> 20°C/ 30 min. (PCR machine)
--> Aliquot 16 μL master mix into DNA
--> Aliquot 20 μL to DNA samples Day 2 (1/26/13) Size Select the Library
--> PCR machine (heat lid 100°C):
--> Zymo clean and concentrator, elute with 15 μL st.dH2O
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