User:Karmella Haynes/Notebook/PcTF Genomics/2013/01/25: Difference between revisions
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{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table --> | {| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table --> | ||
|-valign="top" | |-valign="top" | ||
| <u>Reagent</u> || <u>Volume | | <u>Reagent</u> || <u>Volume</u> | ||
|- | |- | ||
| Input DNA || 30.0 μL | | Input DNA || 30.0 μL | ||
|- | |- | ||
| dH<sub>2</sub>O || 10 | | dH<sub>2</sub>O || 10.0 | ||
|- | |- | ||
| T4 DNA ligase buffer || 5 | | T4 DNA ligase buffer || 5.0 | ||
|- | |- | ||
| dNTP mix || 2 | | dNTP mix || 2.0 | ||
|- | |- | ||
| T4 DNA Polymerase || 1 | | T4 DNA Polymerase || 1.0 | ||
|- | |- | ||
| Klenow DNA polymerase || 1 | | Klenow DNA polymerase || 1.0 | ||
|- | |- | ||
| T4 PNK || 1 | | T4 PNK || 1.0 | ||
|- | |- | ||
| || 50 μL | | || 50 μL | ||
|} | |} | ||
--> 20°C/ 30 min. (PCR machine)<br> | |||
--> 20°C/ 30 min. ( | |||
--> QIAquick PCR Purification, elute with 34 μL EB | --> QIAquick PCR Purification, elute with 34 μL EB | ||
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{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table --> | {| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table --> | ||
|-valign="top" | |-valign="top" | ||
| <u>Reagent</u> || <u>Volume | | <u>Reagent</u> || <u>Volume</u> | ||
|- | |- | ||
| DNA sample || 34.0 μL | | DNA sample || 34.0 μL | ||
|- | |- | ||
| Klenow buffer || 5 | | Klenow buffer || 5.0 | ||
|- | |- | ||
| dATP || 10 | | dATP || 10.0 | ||
|- | |- | ||
| Klenow exo || 1 | | Klenow exo || 1.0 | ||
|- | |- | ||
| || 50 μL | | || 50 μL |
Revision as of 15:13, 25 January 2013
Pc-TF Genomics | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||
01/25/13
Illumina ChIP-seq prep
Perform End Repair
--> 20°C/ 30 min. (PCR machine)
--> Aliquot 16 μL master mix into DNA
--> Aliquot 20 μL to DNA samples |