User:Karmella Haynes/Notebook/PcTF Genomics/2013/01/25: Difference between revisions
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{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table --> | {| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table --> | ||
|-valign="top" | |-valign="top" | ||
| <u>Reagent</u> || <u>Volume | | <u>Reagent</u> || <u>Volume</u> | ||
|- | |- | ||
| DNA sample || 10.0 μL | | DNA sample || 10.0 μL | ||
|- | |- | ||
| DNA ligase buffer || 15 | | DNA ligase buffer || 15.0 | ||
|- | |- | ||
| Adapter oligo mix || 1 | | Adapter oligo mix || 1.0 | ||
|- | |- | ||
| DNA ligase || 4 | | DNA ligase || 4.0 | ||
|- | |- | ||
| || 30 μL | | || 30 μL | ||
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> Elution: 2x 10 μL, add 16 μL st.dH<sub>2</sub>O to eluate (36 μL total) | > Elution: 2x 10 μL, add 16 μL st.dH<sub>2</sub>O to eluate (36 μL total) | ||
[[Image: | [[Image:KAH012613_gel1.tif|300px|Gel purification 01/26/13]] | ||
Revision as of 18:45, 26 January 2013
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01/25/13
Illumina ChIP-seq prep
Perform End Repair
--> 20°C/ 30 min. (PCR machine)
--> Aliquot 16 μL master mix into DNA
--> Aliquot 20 μL to DNA samples Day 2 (1/26/13) Size Select the Library
--> PCR machine (heat lid 100°C):
--> Zymo clean and concentrator, elute with 15 μL st.dH2O
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