Karmella Haynes: /* mm/dd/yy */

Karmella Haynes — Fri, 28 Dec 2012 00:05:13 GMT

mm/dd/yy

←Older revision		Revision as of 00:05, 28 December 2012
Line 8:		Line 8:


-	==mm/dd/yy==	+	==12/27/12==
	<!-- Precede finished items with a checkmark ✓ -->		<!-- Precede finished items with a checkmark ✓ -->
-	* ~~Line item 1~~	+	* PcTF transfection: SK-N-SH TRIzol prep

	----		----
-	'''~~Line item 1~~'''<br>	+	'''SK-N-SH TRIzol prep'''<br>
-	~~> Samples~~	+	# SK-N-SH, 1, +DNA
-		+	# SK-N-SH, 2, (-)
-	~~{\| class="wikitable" border="0" cellspacing="3" <!~~-- ~~Rxn. table -->~~	+
-	~~\|-valign="top"~~	+
-	~~\| <u>Reagent</u> \|\| <u>Volume</u>~~	+
-	\|-	+
-	~~\| reagent~~ 1 ~~\|\| # μL~~	+
-	\|-	+
-	~~\| reagent 2 \|\|~~ #	+
-	\|-	+
-	~~\| reagent 3 \|\| #~~	+
-	\|-	+
-	~~\| reagent 4 \|\| #~~	+
-	\|-	+
-	~~\| dH<sub>~~2~~</sub>O \|\| #~~	+
-	\|-	+
-	~~\|   \|\| # μL~~	+
-	\|}	+
-		+
-	~~--> Reaction conditions~~	+

		+	* Discarded growth medium
		+	* Added 500 μL TRIzol directly to wells
		+	* incubated at r.t. for ~5 min.
		+	* Collected lysed cells from wells with gentle scraping (using 1000 μL pipette)
		+	* Transferred to 2.0 mL tubes. Stored at -80°C

Karmella Haynes: Autocreate 2012/12/27 Entry for User:Karmella_Haynes/Notebook/PcTF_Genomics

Karmella Haynes — Fri, 28 Dec 2012 00:02:13 GMT

Autocreate 2012/12/27 Entry for User:Karmella_Haynes/Notebook/PcTF_Genomics

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]Pc-TF Genomics
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]] {{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|


==mm/dd/yy==

* Line item 1

----
'''Line item 1''' 
> Samples

{| class="wikitable" border="0" cellspacing="3" 
|-valign="top"
| Reagent || Volume
|-
| reagent 1 || # μL
|-
| reagent 2 || #
|-
| reagent 3 || #
|-
| reagent 4 || #
|-
| dH2O || #
|-
|   || # μL
|}

--> Reaction conditions


|}

__NOTOC__

User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/27 - Revision history

Karmella Haynes: /* mm/dd/yy */

Karmella Haynes: Autocreate 2012/12/27 Entry for User:Karmella_Haynes/Notebook/PcTF_Genomics