User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/27: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Pc-TF Genomics</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Pc-TF Genomics</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==mm/dd/yy==
==12/27/12==
<!-- Precede finished items with a checkmark &#x2713; -->
<!-- Precede finished items with a checkmark &#x2713; -->
* Line item 1
* PcTF transfection: SK-N-SH TRIzol prep


----
----
'''Line item 1'''<br>
'''SK-N-SH TRIzol prep'''<br>
> Samples
# SK-N-SH, 1, +DNA
 
# SK-N-SH, 2, (-)
{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table -->
|-valign="top"
| <u>Reagent</u> || <u>Volume</u>
|-
| reagent 1 || # μL
|-
| reagent 2 || #
|-
| reagent 3 || #
|-
| reagent 4 || #
|-
| dH<sub>2</sub>O || #
|-
| &nbsp; || # μL
|}
 
--> Reaction conditions


* Discarded growth medium
* Added 500 μL TRIzol directly to wells
* incubated at r.t. for ~5 min.
* Collected lysed cells from wells with gentle scraping (using 1000 μL pipette)
* Transferred to 2.0 mL tubes. Stored at -80°C




Latest revision as of 22:20, 26 September 2017

Pc-TF Genomics Main project page
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12/27/12

  • PcTF transfection: SK-N-SH TRIzol prep

SK-N-SH TRIzol prep

  1. SK-N-SH, 1, +DNA
  2. SK-N-SH, 2, (-)
  • Discarded growth medium
  • Added 500 μL TRIzol directly to wells
  • incubated at r.t. for ~5 min.
  • Collected lysed cells from wells with gentle scraping (using 1000 μL pipette)
  • Transferred to 2.0 mL tubes. Stored at -80°C



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