User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/12: Difference between revisions

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(Autocreate 2012/12/12 Entry for User:Karmella_Haynes/Notebook/PcTF_Genomics)
 
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==mm/dd/yy==
==12/12/12==
<!-- Precede finished items with a checkmark &#x2713; -->
<!-- Precede finished items with a checkmark &#x2713; -->
* Line item 1
* U2OS: split cells (passage 3)
* SK-N-SH: split cells (passage 3) and set up transfection plate


----
----
'''Line item 1'''<br>
'''U2OS'''<br>
> Samples
* Split cells 1:10, 2x 75 cm flasks


{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table -->
|-valign="top"
| <u>Reagent</u> || <u>Volume</u>
|-
| reagent 1 || # μL
|-
| reagent 2 || #
|-
| reagent 3 || #
|-
| reagent 4 || #
|-
| dH<sub>2</sub>O || #
|-
| &nbsp; || # μL
|}


--> Reaction conditions
----
'''SK-N-SH'''
* Split cells 1:5, 1x 75 cm flask
* Seed 6-well plate for transfection, ~5x10<sup>5</sup> cells/well/1 mL
** Added 3 mL of cells from 10 mL stock flask to 7 mL media; aliquoted 1 mL diluted cells to each well




Revision as of 11:21, 13 December 2012

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12/12/12

  • U2OS: split cells (passage 3)
  • SK-N-SH: split cells (passage 3) and set up transfection plate

U2OS

  • Split cells 1:10, 2x 75 cm flasks


SK-N-SH

  • Split cells 1:5, 1x 75 cm flask
  • Seed 6-well plate for transfection, ~5x105 cells/well/1 mL
    • Added 3 mL of cells from 10 mL stock flask to 7 mL media; aliquoted 1 mL diluted cells to each well



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