User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/12

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(Autocreate 2012/12/12 Entry for User:Karmella_Haynes/Notebook/PcTF_Genomics)
Current revision (14:21, 13 December 2012) (view source)
(12/12/12)
 
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==mm/dd/yy==
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==12/12/12==
<!-- Precede finished items with a checkmark &#x2713; -->
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* Line item 1
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* U2OS: split cells (passage 3)
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* SK-N-SH: split cells (passage 3) and set up transfection plate
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----
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'''Line item 1'''<br>
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'''U2OS'''<br>
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> Samples
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* Split cells 1:10, 2x 75 cm flasks
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{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table -->
 
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|-valign="top"
 
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| <u>Reagent</u> || <u>Volume</u>
 
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|-
 
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| reagent 1 || # μL
 
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|-
 
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| reagent 2 || #
 
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|-
 
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| reagent 3 || #
 
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|-
 
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| reagent 4 || #
 
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|-
 
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| dH<sub>2</sub>O || #
 
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|-
 
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| &nbsp; || # μL
 
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|}
 
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--> Reaction conditions
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----
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'''SK-N-SH'''
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* Split cells 1:5, 1x 75 cm flask
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* Seed 6-well plate for transfection, ~5x10<sup>5</sup> cells/well/1 mL
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** Added 3 mL of cells from 10 mL stock flask to 7 mL media; aliquoted 1 mL diluted cells to each well

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12/12/12

  • U2OS: split cells (passage 3)
  • SK-N-SH: split cells (passage 3) and set up transfection plate



U2OS

  • Split cells 1:10, 2x 75 cm flasks



SK-N-SH

  • Split cells 1:5, 1x 75 cm flask
  • Seed 6-well plate for transfection, ~5x105 cells/well/1 mL
    • Added 3 mL of cells from 10 mL stock flask to 7 mL media; aliquoted 1 mL diluted cells to each well



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