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 Pc-TF Genomics
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12/03/12
- Cell culture tasks: stock cells and Carly's transfection
U2OS
- Froze down 5 vials of U2OS (10% DMSO in Tet-free FBS), using 100% confluent 75 cm flask
- Note: Had Rene transfer from -80°C to -150°C on Thursday
- Seeded 1:10 and 1:20 75 cm flasks from the small back-up flask
SK-N-SH
- Froze down 4 vials of SK-N-SH (10% DMSO in Tet-free FBS), using 90% confluent 75 cm flask
- Note: Had Rene transfer from -80°C to -150°C on Thursday
- Seeded 1:5 75 cm flask from 1/5 of the original 75 cm flask
- These cells don't seem to be very robust
K562
- Seeded 1:10 75 cm flask (passage 5)
K562, Carly's transfection
- Carly observed RFP signal 24 hours after transfection for all three DNA+ samples; no signal in the three negative controls
- Pelleted (individually) each of the 6 samples (from 6-well dish) at 1000 rpm/ 3 min.
- Resuspended in growth medium + pen/strep
- Plated in a total of 10 mL medium + pen/strep in 10 cm petri dishes
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Pc-TF Genomics
