User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/03: Difference between revisions
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== | ==12/03/12== | ||
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* | * Cell culture tasks: stock cells and Carly's transfection | ||
---- | ---- | ||
''' | '''U2OS'''<br> | ||
* Froze down 5 vials of U2OS (10% DMSO in Tet-free FBS), using 100% confluent 75 cm flask | |||
** Note: Had Rene transfer from -80°C to -150°C on Thursday | |||
* Seeded 1:10 and 1:20 75 cm flasks from the small back-up flask | |||
-- | '''SK-N-SH''' | ||
* Froze down 4 vials of SK-N-SH (10% DMSO in Tet-free FBS), using 90% confluent 75 cm flask | |||
** Note: Had Rene transfer from -80°C to -150°C on Thursday | |||
* Seeded 1:5 75 cm flask from 1/5 of the original 75 cm flask | |||
* These cells don't seem to be very robust | |||
'''K562''' | |||
* Seeded 1:10 75 cm flask (passage 5) | |||
'''K562, Carly's transfection''' | |||
* Carly observed RFP signal 24 hours after transfection for all three DNA+ samples; no signal in the three negative controls | |||
* Pelleted (individually) each of the 6 samples (from 6-well dish) at 1000 rpm/ 3 min. | |||
* Resuspended in growth medium + pen/strep | |||
* Plated in a total of 10 mL medium + pen/strep in 10 cm petri dishes | |||
Revision as of 14:28, 8 December 2012
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12/03/12
U2OS
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