User:Karmella Haynes/Notebook/PcTF Genomics/2012/09/21: Difference between revisions
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== | ==09/21/12== | ||
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* | * ChIP-seq: generate enrichment histogram using DNAStar "Array Star" | ||
---- | ---- | ||
''' | '''Enrichment Histogram'''<br> | ||
> | * Start the '''Array Star''' software on the Windows platform | ||
* Open project Pc-TF (note: need to write protocol for creating new ChIP-seq project) | |||
* Open the Peak Table | |||
* Select rows to export | |||
* Choose - Data > Export | |||
** Save to: Browse folder ('''ChIP seq''' on desktop) and type a file name ('''PcTF_092112''') | |||
** Exported Annotation Fields: click Select, choose all options | |||
** (✓) Export Matching Reads, check box | |||
** Reads Files: Browse Documents > DNASTAR > Genome > Downloads > (appropriate gbk file) | |||
** File format: FASTQ (with quality scores) | |||
** (✓) Launch NGen afterwards, check box | |||
* ''Error: Find reads mer matches. Finding matches (141 Mbases, 0.00100 k reads processed). Values exceeded storge bit allocation'' (Close) | |||
* Find new file name.gbk in '''ChIP seq''' | |||
* Start the '''Seq Man NGen''' software | |||
Revision as of 19:53, 1 December 2012
 Pc-TF Genomics
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09/21/12
Enrichment Histogram
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