User:Karmella Haynes/Notebook/PcTF Genomics

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(Cell Type Study)
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==Cell Type Study==
==Cell Type Study==
* Carly Hom - Express PcTF (KAH126) in K-562 and SK-N-SH
* Carly Hom - Express PcTF (KAH126) in K-562 and SK-N-SH
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** K562 - TriZOL prep [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2012/12/18 12/18/12]
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** K562 - TRIzol prep [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2012/12/18 12/18/12]
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** SK-N-SH - TriZOL prep
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** SK-N-SH - TRIzol prep [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2012/12/27 12/27/12]
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** U2OS TriZOL prep
==PcTF-Reporter Activation==
==PcTF-Reporter Activation==

Revision as of 22:56, 26 June 2013

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Illumina ChIP-seq

  • ChIP DNA prep for Illumina:
  • Fasta --> BAM files: Mark Teng from the Dinu lab (TGEN)
  • Enrichment analysis (DNASTAR software)
    • BAM --> peaks/ enrichment histograms workflow 12/13/2012
    • BAM --> wig files for UCSC browser workflow 01/07/2013



Cell Type Study

  • Carly Hom - Express PcTF (KAH126) in K-562 and SK-N-SH

PcTF-Reporter Activation

  • Measure range of PcTF expression in transiently transfected cells - flow cytometry, 06/02/13


Synthetic Repression

  • Gal4-EED/luc staining experiments


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