User:Karmella Haynes/Notebook/BioBrick cloning/Entry Base - Revision history

Karmella Haynes: /* mm/dd/yy */

2012-12-01T21:35:35Z

mm/dd/yy

←Older revision		Revision as of 21:35, 1 December 2012
Line 40:		Line 40:
	# BioBrick name: 5' part/(a/b)/size + 3' part/(c/d)/size		# BioBrick name: 5' part/(a/b)/size + 3' part/(c/d)/size
	# BioBrick name: 5' part/(a/b)/size + 3' part/(c/d)/size		# BioBrick name: 5' part/(a/b)/size + 3' part/(c/d)/size
		+

	* Digests (Fermentas FD)		* Digests (Fermentas FD)

Karmella Haynes: /* mm/dd/yy */

2012-12-01T21:35:14Z

mm/dd/yy

←Older revision		Revision as of 21:35, 1 December 2012
Line 64:		Line 64:


-	> Measure conc.'s	+	* Measure conc.'s
	{\| {{table}} cellspacing="3" <!-- [DNA] table -->		{\| {{table}} cellspacing="3" <!-- [DNA] table -->
	\|- bgcolor=#cfcfcf		\|- bgcolor=#cfcfcf
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-	> Dephosphorylation (Roche)	+	* Dephosphorylation (Roche)
	{\| {{table}} cellspacing="3" <!-- Dephos table -->		{\| {{table}} cellspacing="3" <!-- Dephos table -->
	\|-		\|-
Line 93:		Line 93:


-	> Ligations	+	* Ligations
	{\| {{table}} cellspacing="3" <!-- Ligations table -->		{\| {{table}} cellspacing="3" <!-- Ligations table -->
	\|- bgcolor=#cfcfcf		\|- bgcolor=#cfcfcf

Karmella Haynes: /* mm/dd/yy */

2012-12-01T21:33:11Z

mm/dd/yy

←Older revision		Revision as of 21:33, 1 December 2012
Line 66:		Line 66:
	> Measure conc.'s		> Measure conc.'s
	{\| {{table}} cellspacing="3" <!-- [DNA] table -->		{\| {{table}} cellspacing="3" <!-- [DNA] table -->
-	\|-	+	\|- bgcolor=#cfcfcf
-	\| bgcolor=#cfcfcf \| Sample	+	\| Sample \|\| OD260 \|\| 260/280 \|\| ng/μL
-	\| ~~bgcolor=#cfcfcf~~ \| OD260	+
-	\| ~~bgcolor=#cfcfcf~~ \| 260/280	+
-	\| ~~bgcolor=#cfcfcf~~ \| ng/μL	+
	\|-		\|-
	\| 1. Digested part (a/b) \|\| --- \|\| --- \|\| ---		\| 1. Digested part (a/b) \|\| --- \|\| --- \|\| ---

Karmella Haynes: /* mm/dd/yy */

2012-12-01T21:31:30Z

mm/dd/yy

←Older revision		Revision as of 21:31, 1 December 2012
Line 44:		Line 44:
	** Specific notes		** Specific notes

-	{\| {{table}} ~~border="1"~~ cellspacing="3" <!-- Digest rxn. table -->	+	{\| {{table}} cellspacing="3" <!-- Digest rxn. table -->
	\|- valign="top"		\|- valign="top"
	\| bgcolor=#cfcfcf \| Reagent		\| bgcolor=#cfcfcf \| Reagent
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	> Measure conc.'s		> Measure conc.'s
-	{\| {{~~tabe~~}} ~~border="1"~~ cellspacing="3" <!-- [DNA] table -->	+	{\| {{table}} cellspacing="3" <!-- [DNA] table -->
	\|-		\|-
	\| bgcolor=#cfcfcf \| Sample		\| bgcolor=#cfcfcf \| Sample
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	> Dephosphorylation (Roche)		> Dephosphorylation (Roche)
-	{\| ~~class="wikitable" border="0"~~ cellspacing="3" <!-- Dephos table -->	+	{\| {{table}} cellspacing="3" <!-- Dephos table -->
	\|-		\|-
-	\| ~~<u>~~Reagent~~</u>~~ \|\| ~~<u>~~Volume~~</u>~~	+	\| bgcolor=#cfcfcf \| Reagent
		+	\| bgcolor=#cfcfcf \| Volume
	\|-		\|-
	\| DNA (clean digest) \|\| up to 17 μL (500 ng)		\| DNA (clean digest) \|\| up to 17 μL (500 ng)
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	> Ligations		> Ligations
-	{\| ~~class="wikitable" border="0"~~ cellspacing="3" <!-- Ligations table -->	+	{\| {{table}} cellspacing="3" <!-- Ligations table -->
-	\| ~~<u>~~Ligation~~</u>~~ \|\| <font color="blue"><u>Plate results (lig : neg crtl)</u> mm/dd/yy</font>	+	\|- bgcolor=#cfcfcf
		+	\| Ligation \|\| <font color="blue"><u>Plate results (lig : neg crtl)</u> mm/dd/yy</font>
	\|-		\|-
	\| 1. insert(a/b)/size, ## ng + vector(c/d)/size, ## ng \|\| <font color="blue">new BioBrick #:1 (Pick #)</font>		\| 1. insert(a/b)/size, ## ng + vector(c/d)/size, ## ng \|\| <font color="blue">new BioBrick #:1 (Pick #)</font>
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	\|}		\|}

-	{\| ~~class="wikitable" border="0"~~ cellspacing="3" <!-- Ligation rxn table -->	+	{\| {{table}} cellspacing="3" <!-- Ligation rxn table -->
	\|   \|\| 1 \|\| 2 \|\|		\|   \|\| 1 \|\| 2 \|\|
	\|-		\|-

Karmella Haynes: /* mm/dd/yy */

2012-12-01T21:27:51Z

mm/dd/yy

←Older revision		Revision as of 21:27, 1 December 2012
Line 48:		Line 48:
	\| bgcolor=#cfcfcf \| Reagent		\| bgcolor=#cfcfcf \| Reagent
	\| bgcolor=#cfcfcf \| Volume		\| bgcolor=#cfcfcf \| Volume
-	\| rowspan="9" \| <!-- [[Image:GelImage.jpg\|270px\|Hover name]]<br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] -->	+	\| rowspan="7" \| <!-- [[Image:GelImage.jpg\|270px\|Hover name]]<br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] -->
	\|-		\|-
	\| DNA (plasmid) \|\| up to 25 μL		\| DNA (plasmid) \|\| up to 25 μL

Karmella Haynes: /* mm/dd/yy */

2012-12-01T21:27:04Z

mm/dd/yy

←Older revision		Revision as of 21:27, 1 December 2012
Line 41:		Line 41:
	# BioBrick name: 5' part/(a/b)/size + 3' part/(c/d)/size		# BioBrick name: 5' part/(a/b)/size + 3' part/(c/d)/size

-	> Digests (Fermentas FD)~~<br>~~	+	* Digests (Fermentas FD)
-	~~-->~~ Specific notes~~<br>~~	+	** Specific notes

-	{\| ~~class="wikitable"~~ border="0" cellspacing="3" <!-- Digest rxn. table -->	+	{\| {{table}} border="1" cellspacing="3" <!-- Digest rxn. table -->
-	\|-valign="top"	+	\|- valign="top"
-	\| ~~<u>~~Reagent~~</u>~~ \|\| ~~<u>~~Volume~~</u> \|\|  ~~	+	\| bgcolor=#cfcfcf \| Reagent
		+	\| bgcolor=#cfcfcf \| Volume
	\| rowspan="9" \| <!-- [[Image:GelImage.jpg\|270px\|Hover name]]<br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] -->		\| rowspan="9" \| <!-- [[Image:GelImage.jpg\|270px\|Hover name]]<br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] -->
	\|-		\|-
Line 64:		Line 65:

	> Measure conc.'s		> Measure conc.'s
-	{\| ~~class="wikitable"~~ border="0" cellspacing="3" <!-- [DNA] table -->	+	{\| {{tabe}} border="1" cellspacing="3" <!-- [DNA] table -->
	\|-		\|-
-	\| ~~<u>~~Sample~~</u>~~ \|\| ~~<u>~~OD260~~</u>~~ \|\| ~~<u>~~260/280~~</u>~~ \|\| ~~<u>~~ng/μL~~</u>~~	+	\| bgcolor=#cfcfcf \| Sample
		+	\| bgcolor=#cfcfcf \| OD260
		+	\| bgcolor=#cfcfcf \| 260/280
		+	\| bgcolor=#cfcfcf \| ng/μL
	\|-		\|-
	\| 1. Digested part (a/b) \|\| --- \|\| --- \|\| ---		\| 1. Digested part (a/b) \|\| --- \|\| --- \|\| ---

Karmella Haynes: /* mm/dd/yy */

2012-12-01T21:23:31Z

mm/dd/yy

←Older revision		Revision as of 21:23, 1 December 2012
Line 14:		Line 14:
	----		----
	'''Minipreps'''<br>		'''Minipreps'''<br>
-	> Check with E/P digests	+	* Check with E/P digests

-	{\| ~~class="wikitable"~~ border="0" cellspacing="3" <!-- Digest check rxn. table -->	+	{\| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table -->
-	\|-valign="top"	+	\|- valign="top"
-	\| ~~<u>~~Reagent~~</u>~~ \|\| ~~<u>~~Volume~~</u>~~	+	\| bgcolor=#cfcfcf \| Reagent
		+	\| bgcolor=#cfcfcf \| Volume
	\| rowspan="7" \| <u>Expected:</u><br>1. BB 1 = size<br>2. BB2 = size<br>		\| rowspan="7" \| <u>Expected:</u><br>1. BB 1 = size<br>2. BB2 = size<br>
	\| rowspan="7" \| <!-- [[Image:GelImage.jpg\|400px\|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] -->		\| rowspan="7" \| <!-- [[Image:GelImage.jpg\|400px\|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] -->

Karmella Haynes: /* mm/dd/yy */

2012-07-28T22:34:48Z

mm/dd/yy

←Older revision		Revision as of 22:34, 28 July 2012
Line 10:		Line 10:
	* Line item 1		* Line item 1
	* Line item 2		* Line item 2
		+

	----		----

Karmella Haynes: /* mm/dd/yy */

2012-07-28T22:34:35Z

mm/dd/yy

←Older revision		Revision as of 22:34, 28 July 2012
Line 19:		Line 19:
	\| <u>Reagent</u> \|\| <u>Volume</u>		\| <u>Reagent</u> \|\| <u>Volume</u>
	\| rowspan="7" \| <u>Expected:</u><br>1. BB 1 = size<br>2. BB2 = size<br>		\| rowspan="7" \| <u>Expected:</u><br>1. BB 1 = size<br>2. BB2 = size<br>
-	\| rowspan="7" \| <!-- [[Image:GelImage.jpg\|400px\|Hover name]]<br>15 μL/lane; 1% agarose -->	+	\| rowspan="7" \| <!-- [[Image:GelImage.jpg\|400px\|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] -->
	\|-		\|-
	\| DNA(plasmid) \|\| 2.0 μL		\| DNA(plasmid) \|\| 2.0 μL
Line 45:		Line 45:
	\|-valign="top"		\|-valign="top"
	\| <u>Reagent</u> \|\| <u>Volume</u> \|\|		\| <u>Reagent</u> \|\| <u>Volume</u> \|\|
-	\| rowspan="9" \| <!-- [[Image:GelImage.jpg\|270px\|Hover name]]<br>30 μL/lane, 1% agarose -->	+	\| rowspan="9" \| <!-- [[Image:GelImage.jpg\|270px\|Hover name]]<br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] -->
	\|-		\|-
	\| DNA (plasmid) \|\| up to 25 μL		\| DNA (plasmid) \|\| up to 25 μL

Karmella Haynes at 04:07, 6 July 2012

2012-07-06T04:07:52Z

←Older revision		Revision as of 04:07, 6 July 2012
Line 1:		Line 1:
	{\|{{table}} width="800"		{\|{{table}} width="800"
	\|-		\|-
-	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;"> ~~Project name~~</span>	+	\|style="background-color: #EEE"\|[[Image:owwnotebook_icon.png\|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span>
	\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}		\|style="background-color: #F2F2F2" align="center"\|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}\|0\|-11}}\|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}\|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}\|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
	\|-		\|-

←Older revision		Revision as of 21:27, 1 December 2012
Line 48:		Line 48:
	\| bgcolor=#cfcfcf \| Reagent		\| bgcolor=#cfcfcf \| Reagent
	\| bgcolor=#cfcfcf \| Volume		\| bgcolor=#cfcfcf \| Volume
-	\| rowspan="9" \| <!-- [[Image:GelImage.jpg\|270px\|Hover name]]<br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] -->	+	\| rowspan="7" \| <!-- [[Image:GelImage.jpg\|270px\|Hover name]]<br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] -->
	\|-		\|-
	\| DNA (plasmid) \|\| up to 25 μL		\| DNA (plasmid) \|\| up to 25 μL

←Older revision		Revision as of 22:34, 28 July 2012
Line 19:		Line 19:
	\| <u>Reagent</u> \|\| <u>Volume</u>		\| <u>Reagent</u> \|\| <u>Volume</u>
	\| rowspan="7" \| <u>Expected:</u><br>1. BB 1 = size<br>2. BB2 = size<br>		\| rowspan="7" \| <u>Expected:</u><br>1. BB 1 = size<br>2. BB2 = size<br>
-	\| rowspan="7" \| <!-- [[Image:GelImage.jpg\|400px\|Hover name]]<br>15 μL/lane; 1% agarose -->	+	\| rowspan="7" \| <!-- [[Image:GelImage.jpg\|400px\|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] -->
	\|-		\|-
	\| DNA(plasmid) \|\| 2.0 μL		\| DNA(plasmid) \|\| 2.0 μL
Line 45:		Line 45:
	\|-valign="top"		\|-valign="top"
	\| <u>Reagent</u> \|\| <u>Volume</u> \|\|		\| <u>Reagent</u> \|\| <u>Volume</u> \|\|
-	\| rowspan="9" \| <!-- [[Image:GelImage.jpg\|270px\|Hover name]]<br>30 μL/lane, 1% agarose -->	+	\| rowspan="9" \| <!-- [[Image:GelImage.jpg\|270px\|Hover name]]<br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] -->
	\|-		\|-
	\| DNA (plasmid) \|\| up to 25 μL		\| DNA (plasmid) \|\| up to 25 μL