User:Karmella Haynes/Notebook/BioBrick cloning/Entry Base: Difference between revisions
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> Check with E/P digests | > Check with E/P digests | ||
{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table --> | {| class="wikitable" border="0" cellspacing="3" <!-- Digest check rxn. table --> | ||
|-valign="top" | |-valign="top" | ||
| <u>Reagent</u> || <u>Volume</u> | | <u>Reagent</u> || <u>Volume</u> | ||
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--> Specific notes<br> | --> Specific notes<br> | ||
{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table --> | |||
|- | |||
| <u>Reagent</u> || <u>Volume</u> | |||
|- | |||
| DNA (plasmid) || up to 25 μL | |||
|- | |||
| 10x buffer || 3.0 | |||
|- | |||
| enzyme 1 || 1.0 | |||
|- | |||
| enzyme 2 || 1.0 | |||
|- | |||
| dH<sub>2</sub>O || --- | |||
|- | |||
| || 30 μL --> 37°C/ ~30 min. | |||
|} | |||
Revision as of 07:56, 20 November 2009
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11/18/09
Minipreps
Assemblies
> Digests (Fermentas FD)
Oligo annealing
|