User:Karmella Haynes/Notebook/BioBrick cloning/Entry Base: Difference between revisions
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| <u>Ligation</u> || <font color="blue"><u>Plate results (lig : neg crtl)</u> mm/dd/yy</font> | | <u>Ligation</u> || <font color="blue"><u>Plate results (lig : neg crtl)</u> mm/dd/yy</font> | ||
|- | |- | ||
| 1. insert(a/b)/size, ## ng + vector(c/d)/size, ## ng || <font color="blue">new BioBrick x:y (Pick | | 1. insert(a/b)/size, ## ng + vector(c/d)/size, ## ng || <font color="blue">new BioBrick x:y (Pick #)</font> | ||
|- | |- | ||
| 2. vector(c/d)/ ## ng || | | 2. vector(c/d)/ ## ng || | ||
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{| class="wikitable" border="0" cellspacing="3" <!-- Ligantion rxn table --> | {| class="wikitable" border="0" cellspacing="3" <!-- Ligantion rxn table --> | ||
| || 1 || 2 || | | || 1 || 2 || | ||
|- | |- | ||
| Insert DNA || ### || --- || | | Insert DNA || ### || --- || | ||
|- | |- | ||
| Vector DNA || ### || ### || | | Vector DNA || ### || ### || | ||
|- | |- | ||
| 2x lgn buf (Roche) || ### || ### || | | 2x lgn buf (Roche) || ### || ### || | ||
|- | |- | ||
| T4 ligase (NEB) || 1.0 || 1.0 || | | T4 ligase (NEB) || 1.0 || 1.0 || | ||
|- | |- | ||
| dH<sub>2</sub>O || ### || ### || | | dH<sub>2</sub>O || ### || ### || | ||
|- | |- | ||
| || # μL || # μL || | | || # μL || # μL || |
Revision as of 09:52, 26 November 2009
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
mm/dd/yy
Minipreps
Assemblies
> Digests (Fermentas FD)
Oligo annealing
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