User:Karmella Haynes/Notebook/BioBrick cloning/Entry Base: Difference between revisions
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| <u>Sample</u> || <u>OD260</u> || <u>260/280</u> || <u>ng/μL</u> | | <u>Sample</u> || <u>OD260</u> || <u>260/280</u> || <u>ng/μL</u> | ||
|- | |- | ||
| 1. | | 1. Digested part (a/b) || --- || --- || --- | ||
|- | |- | ||
| 2. | | 2. Digested part (c/d) || --- || --- || --- | ||
|- | |||
|- | |||
|} | |} | ||
Line 76: | Line 64: | ||
> Ligations | |||
> Ligations | {| class="wikitable" border="0" cellspacing="3" <!-- Ligations table --> | ||
{| class="wikitable" border="0" cellspacing="3" | | <u>Ligation</u> || <font color="blue"><u>Plate results (lig : neg crtl)</u> mm/dd/yy</font> | ||
| <u>Ligation</u> || <u>Plate results (lig : neg crtl)</u> | |||
|- | |- | ||
| 1. | | 1. insert(a/b)/size, ## ng + vector(c/d)/size, ## ng || <font color="blue">new BioBrick x:y (Pick 2#)</font> | ||
|- | |- | ||
| 2. | | 2. vector(c/d)/ ## ng || | ||
|} | |} | ||
{| class="wikitable" border="0" cellspacing="3" | {| class="wikitable" border="0" cellspacing="3" <!-- Ligantion rxn table --> | ||
| || 1 || 2 || | | || 1 || 2 || | ||
|- | |- | ||
| Insert DNA || | | Insert DNA || ### || --- || | ||
|- | |- | ||
| Vector DNA || | | Vector DNA || ### || ### || | ||
|- | |- | ||
| 2x lgn buf (Roche) || | | 2x lgn buf (Roche) || ### || ### || | ||
|- | |- | ||
| T4 ligase (NEB) || 1.0 || 1.0 || | | T4 ligase (NEB) || 1.0 || 1.0 || | ||
|- | |- | ||
| dH<sub>2</sub>O || | | dH<sub>2</sub>O || ### || ### || | ||
|- | |- | ||
| || | | || # μL || # μL || | ||
|} | |} | ||
---- | ---- | ||
'''Oligo annealing''' | '''Oligo annealing''' | ||
# | # New BB 1 | ||
# | # New BB 2 | ||
{| class="wikitable" border="0" cellspacing="3" | {| class="wikitable" border="0" cellspacing="3" <!-- Oligo annealing rxn table --> | ||
| DNA (oligos, 100 μM) || up to 18 μL | | DNA (oligos, 100 μM) || up to 18 μL (3 μL ea.) | ||
|- | |- | ||
| 10x annealing buffer || 2.0 | | 10x annealing buffer || 2.0 |
Revision as of 07:33, 20 November 2009
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11/18/09
Assemblies
> Digests (Fermentas FD)
Oligo annealing
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