User:Karmella Haynes/Notebook/BioBrick cloning/2015/08/25: Difference between revisions
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* Re-grow cultures for clones with failed sequencing runs | * Re-grow cultures for clones with failed sequencing runs | ||
* Note: DNASU sampling from the 96-well block is top to bottom, then left to right | * From DNASU Order 10294 | ||
** Note: DNASU sampling from the 96-well block is top to bottom, then left to right | |||
** A1 = L34-001, A2 = L34-002, A3 = L34-003, etc. | ** A1 = L34-001, A2 = L34-002, A3 = L34-003, etc. | ||
* Low phred scores (<700) generally corresponded with the lowqual reads observed in Benchling | * Low phred scores (<700) generally corresponded with the lowqual reads observed in Benchling | ||
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# L34-075, C10, 300 | # L34-075, C10, 300 | ||
# L34-078, F10, 647 | # L34-078, F10, 647 | ||
# L34-080, G10, 495 | |||
# L34-082, B11, 308 | |||
# L34-084, D11, 498 | |||
# L34-093, E12, 8 | |||
# L34-094, F12, 611 | |||
# L34-096, H12, 814 | |||
Revision as of 16:56, 26 August 2015
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08/25/15
CRISPR library 5 mL cultures
Clone, well (agar spot position), phred score
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