User:Karmella Haynes/Notebook/BioBrick cloning/2015/06/23: Difference between revisions
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| bgcolor=#cfcfcf | Mix (x104) | | bgcolor=#cfcfcf | Mix (x104) | ||
| rowspan="7" | Expected:<br>PCR insert/ pJET = ~600 bp<br>1. L34_097_A01<br>2. L34_109_B01<br>3. L34_121_C01<br>4. L34_133_D01<br>5. L34_145_E01<br>6. L34_157_F01<br>7. L34_169_G01<br>8. L34_181_H01 | | rowspan="7" | Expected:<br>PCR insert/ pJET = ~600 bp<br>1. L34_097_A01<br>2. L34_109_B01<br>3. L34_121_C01<br>4. L34_133_D01<br>5. L34_145_E01<br>6. L34_157_F01<br>7. L34_169_G01<br>8. L34_181_H01 | ||
| rowspan="7" | | | rowspan="7" | Gel showed no signal | ||
|- | |- | ||
| Template (culture) || 2.0 || --- | | Template (culture) || 2.0 || --- | ||
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CONCLUSIONS | CONCLUSIONS | ||
* | * Looks like amplification failed | ||
* Move on to sequencing of all clones | |||
* Restart fresh cultures tomorrow | |||
* Will submit as pellets to DNASU | |||
Revision as of 19:05, 24 June 2015
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06/23/15
Cas-tone Assemblies
Thermal cycler: LabNet OptiMax "AnOlig shrt"
RESULTS (6/24/15)
Rene - CRISPR PCR library PCR
1-96. L34_097_A01 - L34_192_H12 (full plate)
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