User:Karmella Haynes/Notebook/BioBrick cloning/2015/06/22: Difference between revisions
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| bgcolor=#cfcfcf | Rxn1-24 | | bgcolor=#cfcfcf | Rxn1-24 | ||
| bgcolor=#cfcfcf | Mix (x104) | | bgcolor=#cfcfcf | Mix (x104) | ||
| rowspan="7" | Expected:<br>PCR insert/ pJET = ~600 bp | | rowspan="7" | Expected:<br>PCR insert/ pJET = ~600 bp<br>1. L34_001_A01<br>2. L34_013_B01<br>3. L34_025_C01<br>4. L34_001_A01 | ||
| rowspan="7" | [[Image: | | rowspan="7" | [[Image:KAH062215_gel1.jpg|250px|Hover name]]<br>5 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|- | |- | ||
| Template (culture) || 2.0 || --- | | Template (culture) || 2.0 || --- | ||
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CONCLUSIONS | CONCLUSIONS | ||
* | * PCR is not as efficient here as it was with the trial run (###) | ||
* Continue with other clones, but program step 1 as 98°C , 10 min. | |||
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| bgcolor=#cfcfcf | Mix (x6) | | bgcolor=#cfcfcf | Mix (x6) | ||
| rowspan=7 | Expected:<br>1. H3-1-40 = 129<br>2. H3-1-60 = 189<br>3. H3-1-80 = 250<br>4. H3-1-116 = 373<br>5. H3-1-136 = 433 | | rowspan=7 | Expected:<br>1. H3-1-40 = 129<br>2. H3-1-60 = 189<br>3. H3-1-80 = 250<br>4. H3-1-116 = 373<br>5. H3-1-136 = 433 | ||
| rowspan=7 | [[Image: | | rowspan=7 | [[Image:KAH062215_gel2.jpg|250px|Hover name]]<br>5 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|- | |- | ||
| Plasmid DNA || 0.2 || 1.2 | | Plasmid DNA || 0.2 || 1.2 |
Revision as of 10:41, 24 June 2015
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||
06/22/15
Rene - CRISPR PCR library PCR
1-96. L34_001_A01 - L34_096_H12 (full plate)
Cas-tone - Phusion PCR for H3
Thermal cycler: Bio-Rad - Phusion
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